Human UCP1 (Mitochondrial brown fat uncoupling protein 1) QuickTest ELISA Kit Packaging:96 wells And then biotinylated Human PTPN22

And then biotinylated Human PTPN22 Antibody is added and binds to PTPN22 in the sample

Then Streptavidin-HRP is added and binds to the Biotinylated MYL3 antibody

If the sample contains RSPO3

Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of NPY in the sample

Human UCP1 (Mitochondrial brown fat uncoupling protein 1) QuickTest ELISA Kit Packaging:96 wells And then biotinylated Human PTPN22The Human UCP1 (Mitochondrial brown fat uncoupling protein 1) QuickTest ELISA Kit measures UCP1 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards