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Then a biotinylated detection antibody specific for Human IL-1Alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
the intensity of color is proportional to the concentration of hGH in the samples
The Rat CCL5/RANTES ELISA kit is a sandwich enzyme immunoassay
Only those wells that contain Porcine IL-2
Canine HCRT (Orexin) ELISA Kit CD144 Then a biotinylated detection antibodyThe Canine (HCRT) Orexin ELISA Kit measures Orexin in Canine samples. The plate has been pre coated with Canine HCRT antibody. HCRT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine HCRT Antibody is added and binds to HCRT in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HCRT antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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