Rat LIF (Leukemia Inhibitory Factor) ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EGFR2

And then biotinylated Rabbit ACAN Antibody is added and binds to ACAN in the sample

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GKN2

Then a biotinylated detection antibody specific for Human IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Rat LIF (Leukemia Inhibitory Factor) ELISA Kit Packaging:96 wells Standards or samples are addedThe Rat (LIF) Leukemia Inhibitory Factor ELISA Kit measures leukemia inhibitory factor in Rat samples. The plate has been pre coated with Rat LIF antibody. LIF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LIF Antibody is added and binds to LIF in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LIF antibody. After incubation unbound Streptavidin HRP is washed away during