Human FZD1 (Frizzled-1) ELISA Kit Packaging:96 wells FABP6 present in the sample

FABP6 present in the sample is added and binds to antibodies coated on the wells

Then Streptavidin-HRP is added and binds to the Biotinylated HSPB1 antibody

Then a biotinylated detection antibody specific for Mouse VEGF-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

and the color intensity is directly proportional to the amount of Human CD40 present

Human FZD1 (Frizzled-1) ELISA Kit Packaging:96 wells FABP6 present in the sampleThe Human (FZD1) Frizzled 1 ELISA Kit measures Frizzled 1 in samples. The plate has been pre coated with Human FZD1 antibody. FZD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FZD1 Antibody is added and binds to FZD1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FZD1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.