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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MCP3
a horseradish peroxidase conjugated mouse anti-Mouse Lymphotactin Antibody is added to the wells and binds to Lymphotactin in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated DNASE2A antibody
only those wells that contain Sheep IL10
Mouse Ear 14 (Eosinophil-associated, ribonuclease A family, member 14) ELISA Kit IL8 Standards or samples are addedThe Mouse Ear 14 (Eosinophil associated, ribonuclease A family, member 14) ELISA Kit measures Ear 14 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Ear14 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound
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