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Substrate solution is then added and color develops in proportion to the amount of Human DKKL1
Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of Apln in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated TGFB1 antibody
If the sample contains TNFSF10
Rat ANG1 (Angiopoietin-1) ELISA Kit CINC-1 Substrate solution is then addedThe Rat ANG1 (Angiopoietin 1) ELISA Kit measures ANG1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ANG 1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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