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Then Streptavidin-HRP is added and binds to the Biotinylated IL-1beta antibody
After incubation unbound Streptavidin- HRP is washed away during a washing step
And then biotinylated Human SPA17 Antibody is added and binds to SPA17 in the sample
TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution
Human CD38 (ADP-ribosyl cyclase 1) QuickTest ELISA Kit L1Ag Then Streptavidin-HRP is added andThe Human CD38 (ADP ribosyl cyclase 1) QuickTest ELISA Kit measures CD38 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into
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