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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL27A
poly(A)+ selection
The gamma subunit is expressed primarily in neurons
Then a biotinylated detection antibody specific for Rat OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Porcine IL-1b ELISA Kit Packaging:48 wells Standards or samples are addedThe Porcine IL 1b ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Pig IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Pig IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those
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