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Sheep T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T
Substrate solution is then added and color develops in proportion to the amount of Human LBP
CRYBA4 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep IL5
Human ANG (Angiogenin) ELISA Kit Packaging:96 wells Sheep T in samples (orThe Human ANG (Angiogenin) ELISA Kit measures ANG in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ANG antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then,
