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CBLN1 present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated GDF10 antibody
The concentration of Mouse EPCAM in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Human KLF15
Human RHO (Rhodopsin) ELISA Kit Packaging:96 wells CBLN1 present in the sampleThe Human (RHO) Rhodopsin ELISA Kit measures Rhodopsin in samples. The plate has been pre coated with Human RHO antibody. RHO present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RHO Antibody is added and binds to RHO in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RHO antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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