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Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light
The concentration of Rat ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve
The concentration of Human GREM2 in the samples is then determined by comparing the OD of the samples to the standard curve
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BAX
Rat ALPI (Alkaline Phosphatase, Intestinal) ELISA Kit (Small Sample) Packaging:48 wells Luciferase catalyzes the reaction: D-fireflyThe Rat ALPI (Alkaline Phosphatase, Intestinal) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat ALPI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat ALPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
