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Substrate solution is then added and color develops in proportion to the amount of Mouse SIRPA
Then a biotinylated detection antibody specific for Human sC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated CXCR3 antibody
a horseradish peroxidase conjugated mouse anti-Human FGFR4 Antibody is added to the wells and binds to FGFR4 in the sample
Rat TWEAK (Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit HLP Substrate solution is then addedThe Rat TWEAK (Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit measures TWEAK in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TWEAK antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound
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