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Then a biotinylated detection antibody specific for Mouse CKM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Calculate the concentration of DEFB124 in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated SEPHS1 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated HSPD1 antibody
Human SERPINH1 (Serpin H1) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human SERPINH1 (Serpin H1) ELISA Kit measures SERPINH1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SERPINH1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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