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Then a biotinylated detection antibody specific for Mouse S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Universal HIS in the sample or standard competes with a fixed amount of Universal HIS on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal HIS
RFP-Tag Rabbit Polyclonal Antibody
and the color intensity is directly proportional to the amount of Human C-Reactive Protein present
Human Beta-Nerve Growth Factor (NGF) ELISA Kit CLGN Then a biotinylated detection antibodyThis ELISA kit is designed to detect Human Beta Nerve Growth Factor (Human NGF). The assay plate has been pre coated with mouse anti Human NGF monoclonal antibody. When the sample containing NGF is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti Human NGF Antibody is added to the wells and binds to NGF in the sample. After washing the wells, substrate solutions are added, and the
