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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CRLF1
And then biotinylated Rat CHRM1 Antibody is added and binds to CHRM1 in the sample
Substrate solution is then added and color develops in proportion to the amount of Rat TNFB
The concentration of Human EEA1 in the samples is then determined by comparing the OD of the samples to the standard curve
Human ZG16B (Zymogen Granule Protein 16 Homolog B) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (ZG16B) Zymogen Granule Protein 16 Homolog B ELISA Kit measures Zymogen Granule Protein 16 Homolog B in samples. The plate has been pre coated with Human ZG16B antibody. ZG16B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ZG16B Antibody is added and binds to ZG16B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ZG16B antibody. After incubation unbound
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