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Calculate the concentration of E2-Ab in the sample by plotting standard curve
Then a biotinylated detection antibody specific for Human MUC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
a horseradish peroxidase conjugated mouse anti-Human ICAM-2 Antibody is added to the wells and binds to ICAM-2 in the sample
A2ML1 present in the sample is added and binds to antibodies coated on the wells
Human PRF1 (Perforin-1) QuickTest ELISA Kit Packaging:96 wells Calculate the concentration of E2-AbThe Human PRF1 (Perforin 1) QuickTest ELISA Kit measures PRF1 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into individual
