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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human FABP3 are added to the micro ELISA plate wells
Bovine Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol
then added a HRP-conjugated antibody specific to Human PGII
Then Streptavidin-HRP is added and binds to the Biotinylated CENPK antibody
Human FAT1 (Protocadherin Fat 1) ELISA Kit Packaging:96 wells Samples (or Standards) and HorseradishThe Human (FAT1) Protocadherin Fat 1 ELISA Kit measures Protocadherin Fat 1 in samples. The plate has been pre coated with Human FAT1 antibody. FAT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FAT1 Antibody is added and binds to FAT1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FAT1 antibody. After incubation unbound Streptavidin HRP is washed away during a
