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and the Human ANXA1 standard plate wells that pre-coated using protein-related techniques are provided separately
coli expression system and the target gene encoding Met1-Asp237 is expressed with a 6His tag at the N-terminus
and the supernatant layer is collected
The concentration of Human OPG in the samples is then determined by comparing the OD of the samples to the standard curve
Mini-sample Mouse VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit Procalcitonin and the Human ANXA1 standardThis Vascular Endothelial Growth Factor Receptor 2 ELISA kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse VEGFR 2 KDR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGFR 2 KDR and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to
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