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Substrate solution is then added and color develops in proportion to the amount of Canine VCAM1
Calculate the concentration of Ppat in the sample by plotting standard curve
The plate has been pre-coated with Rat C1QTNF1 antibody
The concentration of Mouse BMP10 in the samples is then determined by comparing the OD of the samples to the standard curve
Human ITIH4 (Inter-alpha-trypsin Inhibitor Heavy Chain H4) ELISA Kit Mn-SOD Substrate solution is then addedThe Human ITIH4 (Inter alpha trypsin Inhibitor Heavy Chain H4) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human ITIH4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ITIH4 and Avidin Horseradish Peroxidase (HRP) conjugate are added
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