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coli expression system and the target gene encoding Ser78-Ile211 is expressed
a capture antibody-IKZF1-biotin-detection antibody complex was formed
Then a biotinylated detection antibody specific for Rat FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of VEGFR1
Rat LEPR (Leptin Receptor) ELISA Kit XAG-2 coli expression system and theThe Rat LEPR (Leptin Receptor) ELISA Kit measures LEPR in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti LEPR antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
