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The plate has been pre-coated with Human BRAP antibody
Substrate solution is then added and color develops in proportion to the amount of Bovine IGFBP2
Then Streptavidin-HRP is added and binds to the Biotinylated CGN antibody
And then biotinylated Human ATP2A1 Antibody is added and binds to ATP2A1 in the sample
Human OTOL1 (Otolin 1) ELISA Kit Packaging:96 wells The plate has been pre-coatedThe Human (OTOL1) Otolin 1 ELISA Kit measures Otolin 1 in samples. The plate has been pre coated with Human OTOL1 antibody. OTOL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human OTOL1 Antibody is added and binds to OTOL1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated OTOL1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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