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The plate has been pre-coated with Rat STAR antibody
Then Streptavidin-HRP is added and binds to the Biotinylated CHI3L2 antibody
Substrate solution is then added and color develops in proportion to the amount of Mouse GAD1
Then Streptavidin-HRP is added and binds to the Biotinylated CXCL12 antibody
Human DHFR (Dihydrofolate reductase) ELISA Kit Sonic hedgehog The plate has been pre-coatedThe Human DHFR (Dihydrofolate reductase) ELISA Kit measures DHFR in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti DHFR antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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