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Chicken E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2
only those wells that contain Hamster IFNg
Then Streptavidin-HRP is added and binds to the Biotinylated ACAN antibody
it binds with PLN bound to precoated antibody
Rabbit OTR (Oxytocin Receptor) ELISA Kit MCGF Chicken E2 in samples (orThe Rabbit OTR (Oxytocin Receptor) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rabbit OTR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit OTR and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate
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