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The concentration of Rat MC4R in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Rat BetaTG/PBP/CXCL7/NAP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human HLA-DR in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Human CDO1
Human CYP1B1 (Cytochrome P450 1B1) ELISA Kit Packaging:96 wells The concentration of Rat MC4RThe Human (CYP1B1) Cytochrome P450 1B1 ELISA Kit measures Cytochrome P450 1B1 in samples. The plate has been pre coated with Human CYP1B1 antibody. CYP1B1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CYP1B1 Antibody is added and binds to CYP1B1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CYP1B1 antibody. After incubation unbound Streptavidin HRP is washed away
