Leptin ELISA Kit UP-1 a horseradish peroxidase conjugated mouse

a horseradish peroxidase conjugated mouse anti-Human Ephrin-A1 Antibody is added to the wells and binds to Ephrin- A1 in the sample

Only those wells that contain Mouse GM-CSF

You can calculate the concentration of Human SRD5a2 in the samples by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Mouse IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Leptin ELISA Kit UP-1 a horseradish peroxidase conjugated mouseThe Leptin ELISA kit is a two step capture or sandwich type immunoassay to measure Leptin in serum. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for leptin is immobilized onto the microplate and another monoclonal antibody specific for a different epitope of leptin is conjugated to biotin (biotin conjugate). In the first incubation step, leptin present in the specimen samples, calibrators and