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Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of FA/VB9 in the sample
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 44 nm
Calculate the concentration of APOH in the sample by plotting standard curve
REN present in the sample is added and binds to antibodies coated on the wells
Human PKM2 (Pyruvate kinase isozymes M1/M2) ELISA Kit Packaging:96 wells Compare OD450 value of sampleThe Human PKM2 (Pyruvate kinase isozymes M1 M2) ELISA Kit measures PKM2 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PKM2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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