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And then biotinylated Human ENG Antibody is added and binds to ENG in the sample
And then biotinylated Mouse GRIN2B Antibody is added and binds to GRIN2B in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated C8G antibody
The OD value is proportional to the concentration of Human F3/TF
Canine C3A (Complement Component 3a) ELISA Kit Packaging:96 wells And then biotinylated Human ENGThe Canine C3A (Complement Component 3a) ELISA Kit measures C3a in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti C3a antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
