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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TOLLIP
The concentration of Human CD97 in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Human APOL1
The concentration of Human TJP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Mouse NOG (Noggin) ELISA Kit PTX3 Standards or samples are addedThe Mouse (NOG) Noggin ELISA Kit measures Noggin in Mouse samples. The plate has been pre coated with Mouse NOG antibody. NOG present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse NOG Antibody is added and binds to NOG in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NOG antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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