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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Horse E2 are added to the micro ELISA plate wells
You can calculate the concentration of Mouse Klotho in the samples by comparing the OD of the samples to the standard curve
And then biotinylated Human CRNN Antibody is added and binds to CRNN in the sample
Substrate solution is then added and color develops in proportion to the amount of Rat SYT4
Human BLVRB (Biliverdin Reductase B) ELISA Kit Packaging:96 wells Samples (or Standards) and HorseradishThe Human BLVRB (Biliverdin Reductase B) ELISA Kit measures BLVRB in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti BLVRB antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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