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The concentration of Human GDF6 in the samples is then determined by comparing the OD of the samples to the standard curve
NRG2 present in the sample is added and binds to antibodies coated on the wells
The plate has been pre-coated with Human DNAH5 antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BCAM
Human CAF (c-terminal fragment of agrin) ELISA Kit Packaging:96 wells The concentration of Human GDF6The Human CAF (c terminal fragment of agrin) ELISA Kit measures CAF in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CAF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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