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SCT present in the sample is added and binds to antibodies coated on the wells
Then a biotinylated detection antibody specific for Porcine IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Mouse Gm94
APLP2 present in the sample is added and binds to antibodies coated on the wells
Human HRP 2 (Hepatoma-derived growth factor-related protein 2) ELISA Kit Packaging:96 wells SCT present in the sampleThe Human HRP 2 (Hepatoma derived growth factor related protein 2) ELISA Kit measures HRP2 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HRP 2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components.
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