Canine TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit Packaging:48 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NF2

Substrate solution is then added and color develops in proportion to the amount of Human GLI1

it binds with Foxc2 bound to precoated antibody

Bovine E3 in samples (or standards) competes with a fixed amount of E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to E3

Canine TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit Packaging:48 wells Standards or samples are addedThe Canine TNF Alpha (Tumor Necrosis Factor Alpha) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Canine TNF Alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Canine TNF Alpha and Avidin Horseradish Peroxidase (HRP) conjugate are added