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and the color intensity is directly proportional to the amount of Mouse Fetuin A present
Cystatin-3 present in the sample is added and binds to antibodies coated on the wells
Only those wells that contain Human VCAN
The concentration of Mouse MYH7 in the samples is then determined by comparing the OD of the samples to the standard curve
Human 5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit Packaging:96 wells and the color intensity isThe Human 5 HETE (5 Hydroxyeicosatetraenoic Acid) ELISA Kit measures 5 HETE in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 5 HETE. 5 HETE in the sample or standard competes with a fixed amount of biotin labeled 5 HETE for sites on a pre coated antibody specific to 5 HETE. Free components
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