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And then biotinylated Mouse GLI1 Antibody is added and binds to GLI1 in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIF
The concentration of Human LEAP2 in the samples is then determined by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Human TLR1 antibody
Bovine IL-1b (Interleukin 1 Beta) ELISA Kit Serine Proteinase Inhibitor And then biotinylated Mouse GLI1The Bovine IL 1b (Interleukin 1 Beta) ELISA Kit measures IL 1Beta in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 1b antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
