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The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 45 nm
a fixed amount of HRP labeled testosterone competes with the endogenous testosterone in the standard and sample
The concentration of Human DEFa4 in the samples is then determined by comparing the OD of the samples to the standard curve
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNNI3
Rat MUC5AC (Mucin 5 Subtype AC) ELISA Kit (Small Sample) Packaging:96 wells The optical density (OD) isThe Rat MUC5AC (Mucin 5 Subtype AC) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat MUC5AC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat MUC5AC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
