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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL16
only those wells that contain Human PCNT
The plate has been pre-coated with Human CA3 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated ANXA4 antibody
Human HMBS (Porphobilinogen Deaminase) ELISA Kit IFNB Standards or samples are addedThe Human (HMBS) Porphobilinogen Deaminase ELISA Kit measures Porphobilinogen Deaminase in samples. The plate has been pre coated with Human HMBS antibody. HMBS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HMBS Antibody is added and binds to HMBS in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HMBS antibody. After incubation unbound Streptavidin HRP is washed away
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