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Substrate solution is then added and color develops in proportion to the amount of Human FGF-20
the Rat Cys-C (Cystatin C) ELISA kit uses the Sandwich-ELISA principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HGFAC
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TNFSF13
Human GZMH (Granzyme H) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (GZMH) Granzyme H ELISA Kit measures Granzyme H in samples. The plate has been pre coated with Human GZMH antibody. GZMH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GZMH Antibody is added and binds to GZMH in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GZMH antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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