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Substrate solution is then added and color develops in proportion to the amount of Human RAB5B
Mouse Cort in samples (or standards) competes with a fixed amount of Cort on the solid phase supporter for sites on the HRP linked detection antibody specific to Cort
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian IL13
The plate has been pre-coated with Rat SLC22A3 antibody
Human SYT3 (Synaptotagmin 3) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (SYT3) Synaptotagmin 3 ELISA Kit measures Synaptotagmin 3 in samples. The plate has been pre coated with Human SYT3 antibody. SYT3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SYT3 Antibody is added and binds to SYT3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SYT3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
