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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL1a
And then biotinylated Mouse TNNI3K Antibody is added and binds to TNNI3K in the sample
Substrate solution is then added and color develops in proportion to the amount of Porcine LCN1
Then Streptavidin-HRP is added and binds to the Biotinylated SELP antibody
Rat IL-4 (Interleukin 4) ELISA Kit Packaging:96 wells Standards or samples are addedThe Rat (IL 4) Interleukin 4 ELISA Kit measures Interleukin 4 in Rat samples. The plate has been pre coated with Rat IL4 antibody. IL4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat IL4 Antibody is added and binds to IL4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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